DNA-Dependent Protein Kinase Peptide Substrate-汇佰在线平台

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Bright, Long-Lasting Signal for Sensitive Detection of NanoLuc Luciferase

Nano-Glo Luciferase Assay System（Nano-Glo 萤光素酶检测系统）使用简单，仅需一个加样步骤，即能在 NanoLuc 萤光素酶作用下产生辉光型信号，发光信号在常用培养基中的半衰期大约为 120 分钟。该试剂由 Nano-Glo Luciferase Assay Substrate（Nano-Glo萤光素酶检测底物）和 Nano-Glo Luciferase Assay Buffer（Nano-Glo 萤光素酶检测缓冲液）混合而成，并含有完整的裂解缓冲液成分，可以直接用于检测表达 NanoLuc 萤光素酶的细胞或检测分泌了萤光素酶的培养基。_x000D_

Nano-Glo Luciferase Assay Reagent（Nano-Glo 萤光素酶检测试剂）是检测 NanoLuc萤光素酶的专用产品。如需了解有关 NanoLuc萤光素酶的更多细节，请访问NanoLuc Luciferase Technology页面。

A comparison of the sensitivity of NanoLuc, firefly and Renilla luciferase assays. — NanoLuc luciferase is 100X more sensitive than firefly and Renilla luciferase assays.

Assay Advantages

先进的报告基因系统：高亮度NanoLuc报告基因可用于因灵敏度不够而受限的挑战性应用。
简化检测优化过程：“加样 - 读数”模式简化了从实验台操作到高通量筛选的规模放大应用。
改善检测精度：无需分别进行裂解和试剂加样步骤。
信号更亮、更持久：光信号明亮，持续时间更长，为批量和连续处理做了优化。
实现更高的灵敏度：低背景配方提供更高灵敏度。

先进的报告基因系统：高亮 NanoLuc报告基因可以用于因灵敏度不够而受限的挑战性应用。

简化检测优化过程：“加样 - 读数”模式简化了从实验台操作到高通量筛选的规模放大应用。

改善检测精度：无需分别进行裂解和试剂加样步骤。

信号更亮、更持久：光信号明亮，持续时间更长，为批量和连续处理做了优化。

实现更高的灵敏度：低背景配方提供更高灵敏度。

请联系Promega获取更多批量采购或用户配置的信息。

Learn more about custom options for this product at:www.promega.com/custom/
Contact Promega for information on bulk purchases.

Signal decay kinetics for Nano-Glo Luciferase Assay Reagent.

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[ SQL ] SELECT a.name as province,b.name as city FROM `m_brand_region` `br` LEFT JOIN `m_region` `a` ON `a`.`id`=`br`.`provinceId` LEFT JOIN `m_region` `b` ON `b`.`id`=`br`.`cityId` WHERE `br`.`brandId` = 96 [ RunTime:0.001236s ]
[ SQL ] SELECT a.name as province,b.name as city FROM `m_brand_region` `br` LEFT JOIN `m_region` `a` ON `a`.`id`=`br`.`provinceId` LEFT JOIN `m_region` `b` ON `b`.`id`=`br`.`cityId` WHERE `br`.`brandId` = 95 [ RunTime:0.000944s ]
[ SQL ] SELECT a.name as province,b.name as city FROM `m_brand_region` `br` LEFT JOIN `m_region` `a` ON `a`.`id`=`br`.`provinceId` LEFT JOIN `m_region` `b` ON `b`.`id`=`br`.`cityId` WHERE `br`.`brandId` = 94 [ RunTime:0.001094s ]
[ SQL ] SELECT a.name as province,b.name as city FROM `m_brand_region` `br` LEFT JOIN `m_region` `a` ON `a`.`id`=`br`.`provinceId` LEFT JOIN `m_region` `b` ON `b`.`id`=`br`.`cityId` WHERE `br`.`brandId` = 93 [ RunTime:0.001326s ]
[ SQL ] SELECT a.name as province,b.name as city FROM `m_brand_region` `br` LEFT JOIN `m_region` `a` ON `a`.`id`=`br`.`provinceId` LEFT JOIN `m_region` `b` ON `b`.`id`=`br`.`cityId` WHERE `br`.`brandId` = 92 [ RunTime:0.001284s ]
[ SQL ] SELECT a.name as province,b.name as city FROM `m_brand_region` `br` LEFT JOIN `m_region` `a` ON `a`.`id`=`br`.`provinceId` LEFT JOIN `m_region` `b` ON `b`.`id`=`br`.`cityId` WHERE `br`.`brandId` = 91 [ RunTime:0.001179s ]
[ SQL ] SELECT a.name as province,b.name as city FROM `m_brand_region` `br` LEFT JOIN `m_region` `a` ON `a`.`id`=`br`.`provinceId` LEFT JOIN `m_region` `b` ON `b`.`id`=`br`.`cityId` WHERE `br`.`brandId` = 80 [ RunTime:0.001156s ]
[ SQL ] SELECT a.name as province,b.name as city FROM `m_brand_region` `br` LEFT JOIN `m_region` `a` ON `a`.`id`=`br`.`provinceId` LEFT JOIN `m_region` `b` ON `b`.`id`=`br`.`cityId` WHERE `br`.`brandId` = 70 [ RunTime:0.001246s ]
[ SQL ] SELECT a.name as province,b.name as city FROM `m_brand_region` `br` LEFT JOIN `m_region` `a` ON `a`.`id`=`br`.`provinceId` LEFT JOIN `m_region` `b` ON `b`.`id`=`br`.`cityId` WHERE `br`.`brandId` = 69 [ RunTime:0.001293s ]
[ SQL ] SELECT a.name as province,b.name as city FROM `m_brand_region` `br` LEFT JOIN `m_region` `a` ON `a`.`id`=`br`.`provinceId` LEFT JOIN `m_region` `b` ON `b`.`id`=`br`.`cityId` WHERE `br`.`brandId` = 63 [ RunTime:0.001281s ]
[ SQL ] SELECT a.name as province,b.name as city FROM `m_brand_region` `br` LEFT JOIN `m_region` `a` ON `a`.`id`=`br`.`provinceId` LEFT JOIN `m_region` `b` ON `b`.`id`=`br`.`cityId` WHERE `br`.`brandId` = 61 [ RunTime:0.001169s ]
[ SQL ] SELECT a.name as province,b.name as city FROM `m_brand_region` `br` LEFT JOIN `m_region` `a` ON `a`.`id`=`br`.`provinceId` LEFT JOIN `m_region` `b` ON `b`.`id`=`br`.`cityId` WHERE `br`.`brandId` = 58 [ RunTime:0.001272s ]
[ SQL ] SELECT a.name as province,b.name as city FROM `m_brand_region` `br` LEFT JOIN `m_region` `a` ON `a`.`id`=`br`.`provinceId` LEFT JOIN `m_region` `b` ON `b`.`id`=`br`.`cityId` WHERE `br`.`brandId` = 57 [ RunTime:0.001369s ]
[ SQL ] SELECT a.name as province,b.name as city FROM `m_brand_region` `br` LEFT JOIN `m_region` `a` ON `a`.`id`=`br`.`provinceId` LEFT JOIN `m_region` `b` ON `b`.`id`=`br`.`cityId` WHERE `br`.`brandId` = 56 [ RunTime:0.001242s ]
[ SQL ] SELECT a.name as province,b.name as city FROM `m_brand_region` `br` LEFT JOIN `m_region` `a` ON `a`.`id`=`br`.`provinceId` LEFT JOIN `m_region` `b` ON `b`.`id`=`br`.`cityId` WHERE `br`.`brandId` = 55 [ RunTime:0.001261s ]
[ SQL ] SELECT a.name as province,b.name as city FROM `m_brand_region` `br` LEFT JOIN `m_region` `a` ON `a`.`id`=`br`.`provinceId` LEFT JOIN `m_region` `b` ON `b`.`id`=`br`.`cityId` WHERE `br`.`brandId` = 54 [ RunTime:0.001287s ]
[ SQL ] SHOW COLUMNS FROM `m_users` [ RunTime:0.005453s ]
[ SQL ] SELECT * FROM `m_users` WHERE `user_id` = 0 LIMIT 1 [ RunTime:0.002112s ]
[ SQL ] SHOW COLUMNS FROM `m_wx_user` [ RunTime:0.003084s ]
[ SQL ] SELECT `qr` FROM `m_wx_user` LIMIT 1 [ RunTime:0.001217s ]
[ SQL ] SHOW COLUMNS FROM `m_goods` [ RunTime:0.008691s ]
[ SQL ] SELECT * FROM `m_goods` WHERE `goods_id` = 1419 LIMIT 1 [ RunTime:0.004589s ]
[ SQL ] UPDATE `m_goods` SET `click_count`=15 WHERE `goods_id` = 1419 [ RunTime:0.027039s ]
[ SQL ] SELECT `goods_id`,`brand_id`,`goods_name`,`market_price`,`generalMemberPrice`,`agentPrice` FROM `m_goods` WHERE `goods_id` <> 1419 AND `cat_id` <> 542 AND `is_on_sale` = 1 AND `exchange_integral` = 0 AND `is_virtual` = 0 ORDER BY click_count desc LIMIT 0,12 [ RunTime:0.202725s ]
[ SQL ] SELECT `goods_id`,`brand_id`,`goods_name`,`market_price`,`generalMemberPrice`,`agentPrice` FROM `m_goods` WHERE `is_hot` = 1 AND `is_on_sale` = 1 AND `exchange_integral` = 0 AND `is_virtual` = 0 ORDER BY goods_id desc LIMIT 0,10 [ RunTime:0.001528s ]
[ SQL ] SHOW COLUMNS FROM `m_config` [ RunTime:0.003666s ]
[ SQL ] SELECT `value` FROM `m_config` WHERE `name` = 'askConfig' LIMIT 1 [ RunTime:0.001179s ]
[ SQL ] SELECT `br`.*,a.name as province,b.name as city FROM `m_brand_region` `br` LEFT JOIN `m_region` `a` ON `a`.`id`=`br`.`provinceId` LEFT JOIN `m_region` `b` ON `b`.`id`=`br`.`cityId` WHERE `br`.`brandId` = 55 [ RunTime:0.001114s ]
[ SQL ] SELECT `id`,`name`,`supplyCycle` FROM `m_brand` WHERE `id` = 55 LIMIT 1 [ RunTime:0.001086s ]
[ SQL ] SHOW COLUMNS FROM `m_after_sales_service` [ RunTime:0.002344s ]
[ SQL ] SELECT * FROM `m_after_sales_service` `a` LEFT JOIN `m_after_sales_goods` `g` ON `a`.`id`=`g`.`after_sales_id` WHERE `goods_id` = 1419 [ RunTime:0.001083s ]
[ SQL ] SHOW COLUMNS FROM `m_goods_category` [ RunTime:0.001949s ]
[ SQL ] SELECT * FROM `m_goods_category` WHERE `id` = 542 LIMIT 1 [ RunTime:0.001376s ]
[ SQL ] SELECT * FROM `m_goods_category` WHERE `id` IN (530,536) [ RunTime:0.001406s ]
[ SQL ] SELECT * FROM `m_goods_category` WHERE `id` IN (530,536) [ RunTime:0.001110s ]
[ SQL ] SELECT * FROM `m_goods_category` WHERE `id` IN (530,536) [ RunTime:0.001152s ]
[ SQL ] SELECT `original_img` FROM `m_goods` WHERE `goods_id` = 1419 LIMIT 1 [ RunTime:0.001087s ]
[ SQL ] SHOW COLUMNS FROM `m_goods_images` [ RunTime:0.002212s ]
[ SQL ] SELECT * FROM `m_goods_images` WHERE `goods_id` = 1419 ORDER BY `img_id` [ RunTime:0.001185s ]
[ SQL ] SHOW COLUMNS FROM `m_ad_goods` [ RunTime:0.002462s ]
[ SQL ] SELECT * FROM `m_ad_goods` `g` LEFT JOIN `m_ad_goods_config` `c` ON `c`.`ad_goods_id`=`g`.`ad_goods_id` WHERE ( g.enabled=1 AND g.end_time > 1746019720 AND g.start_time < 1746019720 and(c.goods_id=1419 OR c.goods_id is null) ) GROUP BY `c`.`goods_id` ORDER BY c.goods_id asc,g.orderby desc LIMIT 1 [ RunTime:0.001117s ]
[ SQL ] SELECT * FROM `m_ad_goods` `g` LEFT JOIN `m_ad_goods_config` `c` ON `c`.`ad_goods_id`=`g`.`ad_goods_id` WHERE ( g.enabled=1 AND g.end_time > 1746019720 AND g.start_time < 1746019720 and(c.goods_id=1419 OR c.goods_id is null) ) GROUP BY `c`.`goods_id` ORDER BY c.goods_id asc,g.orderby desc LIMIT 1 [ RunTime:0.001057s ]
[ SQL ] SELECT * FROM `m_ad_goods` `g` LEFT JOIN `m_ad_goods_config` `c` ON `c`.`ad_goods_id`=`g`.`ad_goods_id` WHERE ( g.enabled=1 AND g.end_time > 1746019720 AND g.start_time < 1746019720 and(c.goods_id=1419 OR c.goods_id is null) ) GROUP BY `c`.`goods_id` ORDER BY c.goods_id asc,g.orderby desc LIMIT 1 [ RunTime:0.001062s ]
[ SQL ] SHOW COLUMNS FROM `m_comment` [ RunTime:0.002225s ]
[ SQL ] SELECT sum(case when img !='' and img not like 'N;%' then 1 else 0 end) as img_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)= 4 or ceil((deliver_rank+goods_rank+service_rank)/3)= 5 then 1 else 0 end) as high_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)= 3 then 1 else 0 end) as center_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)<=2 then 1 else 0 end) as low_sum,count(comment_id) as total_sum FROM `m_comment` WHERE `is_show` = 1 AND `goods_id` = 1419 AND `username` <> 'admin' GROUP BY `goods_id` LIMIT 1 [ RunTime:0.001325s ]
[ SQL ] SHOW COLUMNS FROM `m_askall_question` [ RunTime:0.002283s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `m_askall_question` WHERE `goods_id` = 1419 AND `status` = 1 AND ( `audit` = 1 ) LIMIT 1 [ RunTime:0.001148s ]
[ SQL ] SHOW COLUMNS FROM `m_goods_attr` [ RunTime:0.001849s ]
[ SQL ] SELECT * FROM `m_goods_attr` INNER JOIN `m_goods_attribute` `b` ON `m_goods_attr`.`attr_id`=`b`.`attr_id` WHERE `goods_id` = 1419 ORDER BY b.order desc [ RunTime:0.001577s ]
[ SQL ] SELECT sum(case when img !='' and img not like 'N;%' then 1 else 0 end) as img_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)= 4 or ceil((deliver_rank+goods_rank+service_rank)/3)= 5 then 1 else 0 end) as high_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)= 3 then 1 else 0 end) as center_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)<=2 then 1 else 0 end) as low_sum,count(comment_id) as total_sum FROM `m_comment` WHERE `is_show` = 1 AND `goods_id` = 1419 AND `username` <> 'admin' GROUP BY `goods_id` LIMIT 1 [ RunTime:0.001314s ]
[ SQL ] SELECT sum(case when img !='' and img not like 'N;%' then 1 else 0 end) as img_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)= 4 or ceil((deliver_rank+goods_rank+service_rank)/3)= 5 then 1 else 0 end) as high_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)= 3 then 1 else 0 end) as center_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)<=2 then 1 else 0 end) as low_sum,count(comment_id) as total_sum FROM `m_comment` WHERE `is_show` = 1 AND `goods_id` = 1419 AND `username` <> 'admin' GROUP BY `goods_id` LIMIT 1 [ RunTime:0.001289s ]
[ SQL ] SELECT sum(case when img !='' and img not like 'N;%' then 1 else 0 end) as img_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)= 4 or ceil((deliver_rank+goods_rank+service_rank)/3)= 5 then 1 else 0 end) as high_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)= 3 then 1 else 0 end) as center_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)<=2 then 1 else 0 end) as low_sum,count(comment_id) as total_sum FROM `m_comment` WHERE `is_show` = 1 AND `goods_id` = 1419 AND `username` <> 'admin' GROUP BY `goods_id` LIMIT 1 [ RunTime:0.001423s ]
[ SQL ] SELECT sum(case when img !='' and img not like 'N;%' then 1 else 0 end) as img_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)= 4 or ceil((deliver_rank+goods_rank+service_rank)/3)= 5 then 1 else 0 end) as high_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)= 3 then 1 else 0 end) as center_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)<=2 then 1 else 0 end) as low_sum,count(comment_id) as total_sum FROM `m_comment` WHERE `is_show` = 1 AND `goods_id` = 1419 AND `username` <> 'admin' GROUP BY `goods_id` LIMIT 1 [ RunTime:0.001384s ]
[ SQL ] SELECT sum(case when img !='' and img not like 'N;%' then 1 else 0 end) as img_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)= 4 or ceil((deliver_rank+goods_rank+service_rank)/3)= 5 then 1 else 0 end) as high_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)= 3 then 1 else 0 end) as center_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)<=2 then 1 else 0 end) as low_sum,count(comment_id) as total_sum FROM `m_comment` WHERE `is_show` = 1 AND `goods_id` = 1419 AND `username` <> 'admin' GROUP BY `goods_id` LIMIT 1 [ RunTime:0.001450s ]
[ SQL ] SELECT sum(case when img !='' and img not like 'N;%' then 1 else 0 end) as img_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)= 4 or ceil((deliver_rank+goods_rank+service_rank)/3)= 5 then 1 else 0 end) as high_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)= 3 then 1 else 0 end) as center_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)<=2 then 1 else 0 end) as low_sum,count(comment_id) as total_sum FROM `m_comment` WHERE `is_show` = 1 AND `goods_id` = 1419 AND `username` <> 'admin' GROUP BY `goods_id` LIMIT 1 [ RunTime:0.001353s ]
[ SQL ] SELECT sum(case when img !='' and img not like 'N;%' then 1 else 0 end) as img_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)= 4 or ceil((deliver_rank+goods_rank+service_rank)/3)= 5 then 1 else 0 end) as high_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)= 3 then 1 else 0 end) as center_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)<=2 then 1 else 0 end) as low_sum,count(comment_id) as total_sum FROM `m_comment` WHERE `is_show` = 1 AND `goods_id` = 1419 AND `username` <> 'admin' GROUP BY `goods_id` LIMIT 1 [ RunTime:0.001234s ]
[ SQL ] SELECT sum(case when img !='' and img not like 'N;%' then 1 else 0 end) as img_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)= 4 or ceil((deliver_rank+goods_rank+service_rank)/3)= 5 then 1 else 0 end) as high_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)= 3 then 1 else 0 end) as center_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)<=2 then 1 else 0 end) as low_sum,count(comment_id) as total_sum FROM `m_comment` WHERE `is_show` = 1 AND `goods_id` = 1419 AND `username` <> 'admin' GROUP BY `goods_id` LIMIT 1 [ RunTime:0.001429s ]
[ SQL ] SELECT sum(case when img !='' and img not like 'N;%' then 1 else 0 end) as img_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)= 4 or ceil((deliver_rank+goods_rank+service_rank)/3)= 5 then 1 else 0 end) as high_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)= 3 then 1 else 0 end) as center_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)<=2 then 1 else 0 end) as low_sum,count(comment_id) as total_sum FROM `m_comment` WHERE `is_show` = 1 AND `goods_id` = 1419 AND `username` <> 'admin' GROUP BY `goods_id` LIMIT 1 [ RunTime:0.001449s ]
[ SQL ] SELECT sum(case when img !='' and img not like 'N;%' then 1 else 0 end) as img_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)= 4 or ceil((deliver_rank+goods_rank+service_rank)/3)= 5 then 1 else 0 end) as high_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)= 3 then 1 else 0 end) as center_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)<=2 then 1 else 0 end) as low_sum,count(comment_id) as total_sum FROM `m_comment` WHERE `is_show` = 1 AND `goods_id` = 1419 AND `username` <> 'admin' GROUP BY `goods_id` LIMIT 1 [ RunTime:0.001331s ]
[ SQL ] SELECT sum(case when img !='' and img not like 'N;%' then 1 else 0 end) as img_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)= 4 or ceil((deliver_rank+goods_rank+service_rank)/3)= 5 then 1 else 0 end) as high_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)= 3 then 1 else 0 end) as center_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)<=2 then 1 else 0 end) as low_sum,count(comment_id) as total_sum FROM `m_comment` WHERE `is_show` = 1 AND `goods_id` = 1419 AND `username` <> 'admin' GROUP BY `goods_id` LIMIT 1 [ RunTime:0.001351s ]
[ SQL ] SELECT sum(case when img !='' and img not like 'N;%' then 1 else 0 end) as img_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)= 4 or ceil((deliver_rank+goods_rank+service_rank)/3)= 5 then 1 else 0 end) as high_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)= 3 then 1 else 0 end) as center_sum,sum(case when ceil((deliver_rank+goods_rank+service_rank)/3)<=2 then 1 else 0 end) as low_sum,count(comment_id) as total_sum FROM `m_comment` WHERE `is_show` = 1 AND `goods_id` = 1419 AND `username` <> 'admin' GROUP BY `goods_id` LIMIT 1 [ RunTime:0.002921s ]
[ SQL ] select * from `m_friend_link` where is_show=1 [ RunTime:0.004154s ]
[ SQL ] SELECT * FROM `m_navigation` where is_show = 1 AND position = 'bottom' ORDER BY `sort` DESC [ RunTime:0.002425s ]

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DNA-Dependent Protein Kinase Peptide Substrate

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